Báo cáo khóa học: Conformational changes of b-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles A fluorescence and CD study

Conformational Changes of β-Lactoglobulin in Sodium Bis(2-ethylhexyl) Sulfosuccinate Reverse Micelles

Authors:

Suzana M. Andrade, Teresa I. Carvalho, M. Isabel Viseu, and Sílvia M. B. Costa

Field:

Biochemistry, Biophysics

Document Content:

This document presents a study on the conformational changes of β-lactoglobulin (BLG) when encapsulated in sodium bis(2-ethylhexyl) sulfosuccinate (AOT) reverse micelles (RM). The research utilizes fluorescence and circular dichroism (CD) spectroscopy to investigate how varying water content (ω₀) within the RM affects the protein’s structure and the environment of its tryptophan (Trp) residues. The findings suggest significant alterations in BLG’s tertiary structure and a distortion of its β-sheet conformation as the water content increases. The study also explores the accessibility of Trp residues to quenchers like acrylamide and CCl₄ to further understand these structural modifications and the protein’s location within the micellar system.

Detailed Table of Contents:

• Introduction
• Materials and Methods
• Results
• CD spectra of BLG in AOT RM
• Fluorescence of BLG in AOT RM
• Fluorescence quenching of Trp residues in BLG
• Discussion
• CD data
• Fluorescence data
• BLG location on AOT RM
• Encapsulation effect on the Trp environment
• Conclusions
• Acknowledgements
• References