Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley

Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley

Authors: Tomas Morosinotto, Roberto Bassi, Sara Frigerio, Giovanni Finazzi, Edward Morris, and James Barber

Field: Plant Biochemistry and Biophysics

Document Content: This study investigates the biochemical and structural characteristics of Photosystem II (PSII) supercomplexes in a barley mutant (‘viridis zb63’) that lacks Photosystem I (PSI). This mutation leads to chronic over-reduction of the plastoquinone pool, mimicking conditions of extreme light stress in wild-type plants. The research elucidates the minimal PSII antenna unit, its structural organization, and its implications for photoprotection and energy regulation. The findings reveal distinct signaling pathways involved in antenna size regulation and carotenoid biosynthesis, highlighting the crucial role of the plastoquinone redox state.

Detailed Table of Contents:

• Introduction
• Keywords
• Correspondence
• Abbreviations
• Abstract
• Introduction
• Results: The PQ pool is over-reduced in viridis zb63
• Results: Antenna size is not regulated in viridis zb63
• Discussion: The PQ redox state is a key signal for antenna size regulation but not for carotenoid biosynthesis
• Structural characterization of minimal PSII antenna unit
• The physiological relevance of PSII array formation
• Experimental procedures
• Biochemistry
• EM
• Image analysis
• Molecular modelling
• Acknowledgements
• References