Xem trước tài liệu

Đang tải tài liệu...

Thông tin chi tiết tài liệu

Định dạng: PDF
Số trang: 10 trang
Dung lượng: 238 KB

Giới thiệu nội dung

Acharan sulfate, the new glycosaminoglycan from Achatina fulica Bowdich 1822

Tác giả: Tuane C. R. G. Vieira, Adilson Costa-Filho, Norma C. Salgado, Silvana Allodi, Ana-Paula Valente, Luiz E. Nasciutti and Luiz-Claudio F. Silva

Lĩnh vực: Biochemistry, Glycosaminoglycans, Snail Biology

Nội dung tài liệu: This document details the structural characterization, metabolic labeling, and localization of acharan sulfate, a novel glycosaminoglycan discovered in the giant African snail, Achatina fulica. The research elucidates the heterogeneity and compartmental distribution of acharan sulfate within the snail’s body, mucus, and organic shell matrix. It also investigates its structural relationship to heparin and heparan sulfate and explores potential biological roles. The study employs various biochemical and histochemical techniques, including chromatography, electrophoresis, enzymatic digestion, and radiolabeling, to provide comprehensive insights into this GAG.

Mục lục chi tiết:

  • Acharan sulfate, the new glycosaminoglycan from Achatina fulica Bowdich 1822
  • Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix
  • Keywords: acharan sulfate; Achatina fulica, glycosaminoglycans; mucus; organic shell matrix; snail.
  • Introduction
  • Materials
  • Experimental procedures
  • Preparation of GAGs from the soft body
  • Collection and analysis of mucus GAGs
  • Extraction of GAGs from the organic shell matrix
  • In vivo metabolic 35S-labeling of snail body GAGs
  • Isolation of the radiolabeled GAGs
  • Identification of the snail GAGs
  • Agarose gel electrophoresis
  • Digestion with chondroitin lyases
  • Digestion with heparin lyase I
  • Deamination with nitrous acid
  • N-acetylation of purified GAG fractions
  • Polyacrylamide gel electrophoresis
  • Superose-12 HPLC gel filtration chromatography
  • β-elimination of GAGs from mucus
  • NMR spectroscopic analysis
  • Radioautographic and metachromatic detection of sulfated GAGs
  • Results
  • Heterogenic forms of acharan sulfate are present in the soft body, mucus and the organic shell matrix
  • Acharan sulfate appears to exist as a protein-free polysaccharide in mucus
  • In vivo metabolic labeling of acharan sulfate
  • Acharan sulfate is widely distributed in the snail body
  • Discussion
  • Acknowledgements
  • References