Báo cáo khoa học: The intrinsic structure of glucose transporter isoforms Glut1 and Glut3 regulates their differential distribution to detergent-resistant membrane domains in nonpolarized mammalian cells

The Intrinsic Structure Of Glucose Transporter Isoforms Glut1 And Glut3 Regulates Their Differential Distribution To Detergent-Resistant Membrane Domains In Nonpolarized Mammalian Cells

Authors: Tomoko Sakyo, Hiroaki Naraba, Hirobumi Teraoka, Takayuki Kitagawa

Field: Biochemistry, Cell Biology, Molecular Pathology

Document Summary: This study investigates the structural basis for the differential distribution of glucose transporter 1 (Glut1) and glucose transporter 3 (Glut3) in nonpolarized mammalian cells. Previously, Glut1 was found in detergent-resistant membrane (DRM) domains, while Glut3 was located in fluid membrane domains. Through the analysis of chimeric constructs, researchers aimed to identify specific regions responsible for this segregation. The findings suggest that while neither the N-terminal nor the C-terminal cytosolic tails alone determine this distribution, a critical sorting signal may reside within the N-terminal half of the membrane-spanning segments. Alterations in these regions significantly impacted the distribution, indicating that a substantial portion of the intrinsic structure, including the N-terminus, plays a role in regulating this process. The study contributes to understanding how these isoforms are localized within distinct membrane domains, potentially influencing their functions.

Detailed Table of Contents: (Extracted from document sections)

• Introduction
• Keywords
• Correspondence
• Abstract
• Results
• Expression and DRM distribution of FLAG-tagged Glut1 (FG1) and FLAG-tagged Glut3 (FG3)
• Glucose uptake by CHO cells transfected with FG1 and FG3
• Neither the N-terminus nor the C-terminus of Glut1 is needed for the DRM distribution
• The N-terminal membrane-spanning regions of Glut1 are needed for the DRM distribution
• Role of a large intracellular loop of Glut1 in the DRM distribution
• Discussion
• Experimental procedures
• Antibodies and reagents
• Plasmids
• Cell culture and transfection
• Immunofluorescence analysis
• Detergent solubilization and immunoblotting
• [H]2DG uptake assays
• Acknowledgements
• References