Xem trước tài liệu

Đang tải tài liệu...

Thông tin chi tiết tài liệu

Định dạng: PDF
Số trang: 10 trang
Dung lượng: Đang cập nhật

Giới thiệu nội dung

Saccharomyces cerevisiae Cox18 complements the essential Sec-independent function of Escherichia coli YidC

Authors: Edwin van Bloois, Gregory Koningstein, Heike Bauerschmitt, Johannes M. Herrmann, Joen Luirink

Field: Molecular Microbiology, Cell Biology, Physiological Chemistry

Document Content:

This study investigates the role of Saccharomyces cerevisiae Cox18 in the context of membrane protein biogenesis. The YidC/Oxa1/Alb3 protein family is crucial for the assembly of membrane proteins in bacteria, mitochondria, and chloroplasts. In Escherichia coli, YidC is essential for integrating and assembling numerous inner membrane proteins, functioning both with and independently of the Sec-translocon. Higher eukaryotes have two homologous proteins, Oxa1 and Cox18/Oxa2. Oxa1 is vital for inserting membrane proteins into the mitochondrial inner membrane, while Cox18/Oxa2 has a less defined role in the cytochrome c oxidase complex biogenesis. Through a genetic complementation approach, expressing the conserved region of yeast Cox18 in E. coli, this research demonstrates that Cox18 can indeed complement the essential Sec-independent function of YidC. This finding establishes Cox18 as a bona fide member of the YidC/Oxa1/Alb3 family, highlighting a conserved insertase/translocase activity. The study further explores the implications of this conserved function across different organisms and pathways involved in membrane protein biogenesis.

Detailed Table of Contents:

  • Introduction
  • Keywords
  • Correspondence
  • Abstract
  • Abbreviations
  • Members of the YidC/Oxa1/Alb3 protein family
  • Conserved mechanisms in membrane protein biogenesis
  • The role of YidC
  • Results: Properties of YidC, Oxa1 and Cox18
  • EcCox18 complements the growth defect of a YidC depletion strain
  • EcCox18 functions as a Sec-independent insertase
  • EcCox18 is not cross-linked to nascent chains of Sec-dependent IMPs
  • Discussion
  • Experimental procedures
  • Acknowledgements
  • References