Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines

Interaction of G-rich GT oligonucleotides with nuclear-associated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines

Authors: Bruna Scaggiante, Barbara Dapas, Gabriele Grassi, Giorgio Manzini

Field: Biochemistry, Biophysics and Macromolecular Chemistry; Clinical, Morphological, and Technological Sciences, Division of Internal Medicine

Document Content: This study investigates the antiproliferative effects of G-rich GT oligonucleotides (GTs) on human T-lymphoblast CCRF-CEM cancer cells. The research explores the correlation between the ability of these oligomers to form G-quadruplex structures, their binding to nuclear-associated proteins, specifically eEF1A, and their resulting cytotoxicity. The findings suggest that the antiproliferative activity is directly linked to the binding affinity of GT oligomers to eEF1A, and this effect is inversely related to the oligomers’ capacity to adopt G-quadruplex structures under physiological conditions. Oligomers that do not form G-quadruplexes or have poor structures exhibit a significant cellular growth inhibition effect, which is associated with their binding to eEF1A.

Detailed Table of Contents:

• Keywords
• Correspondence
• Abstract
• Introduction
• Results
• Discussion
• Experimental Procedures
• Acknowledgments
• References