Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides

Tios T-te – A Prototypical Thioesterase Responsible for Cyclodimerization of the Quinoline- and Quinoxaline-type Class of Chromodepsipeptides

Authors: Lars Robbel, Katharina M. Hoyer, Mohamed A. Marahiel

Field: Biochemistry / Chemistry

Document Content: This study presents the first in vitro characterization of TioS T-TE, a thioesterase enzyme crucial for the iterative assembly of quinoline- and quinoxaline-type chromodepsipeptides. The research details the enzyme’s capability to catalyze both macrolactonization and macrothiolactonization. It explores the substrate specificity of TioS T-TE and optimizes macrocyclization reactions to achieve maximum yields. The study confirms the backward mechanism proposed for iterative thioesterases and investigates chemoenzymatically generated macrocycles for their DNA-bisintercalation activity. TioS T-TE is highlighted as a robust and versatile catalyst for producing chromodepsipeptide analogs, potentially leading to compounds with enhanced pharmaceutical properties.

Detailed Table of Contents:

• Keywords
• Correspondence
• Abstract
• Introduction
• Results
• Discussion
• Experimental Procedures
• Acknowledgements
• References
• Supporting Information