Báo cáo khoa học: Identification of novel fibronectin fragments detected specifically in juvenile urine

Identification of Novel Fibronectin Fragments Detected Specifically in Juvenile Urine

Authors: Reiko Iida, Toshihiro Yasuda, and Koichiro Kishi

Field: Forensic Medicine, Medical Genetics and Biochemistry, Legal Medicine

Document Content: This study focuses on the identification and characterization of novel fibronectin fragments, designated Ugl-Y, found specifically in the urine of young individuals. Through extensive biochemical analysis, including protein sequencing and mass spectrometry, researchers determined that Ugl-Y fragments are derived from fibronectin, a major component of the extracellular matrix. Specifically, Ugl-Y fragments Y1 and Y2 were identified as corresponding to regions A723-R911 and A723-R903 of human fibronectin, respectively. The research suggests that Ugl-Y is likely produced by the proteolytic degradation of fibronectin, potentially occurring during the process of vigorous bone resorption in children and adolescents. The findings indicate that Ugl-Y may serve as a novel biomarker for age-related changes and bone absorption.

Detailed Table of Contents:

• Identification of novel fibronectin fragments detected specifically in juvenile urine
• Keywords
• Correspondence
• Abstract (Ugl-Y (young age-related urinary glycoprotein) is an age-specific protein…)
• Abbreviations
• Figure 1. IEF-PAGE analysis (pH 2.5-5) of urine samples…
• Figure 2. HPLC separation of tryptic (A), chymotryptic (B) and endoproteinase Asp-N (C) peptides…
• Figure 3. Amino acid sequence of Ugl-Y in comparison with that of human fibronectin (A723-R911)…
• Analysis of Y1 (ATSESVTEITASSFVVSW) with the protein database (BLASTP)…
• Figure 4. IEF-PAGE analysis (pH 2.5-5) of cell lysates prepared from Ugl-Y-expressing cells.
• Figure 5. Detection of Ugl-Y from pediatric serum.
• Figure 6. Digestion of FN by osteoclast cell extracts.
• Figure 7. Positional relationships between Ugl-Y and FN.
• Discussion
• Expression of Ugl-Y in COS-7 cells
• Digestion of FN by osteoclast cell extracts
• Experimental procedures
• Biological samples
• Purification of Ugl-Y from pediatric urine
• Purification of Ugl-Y from pediatric serum
• Electrophoresis
• N-terminal amino acid sequence analysis of Ugl-Y
• Internal amino acid sequence analysis of Ugl-Y
• MALDI-TOF MS
• Construction of expression vectors and expression in COS-7 cells
• Acknowledgements
• References