Báo cáo khoa học: An investigation into the effect of potassium ions on the folding of silk fibroin studied by generalized two-dimensional NMR–NMR correlation and Raman spectroscopy

An Investigation Into The Effect Of Potassium Ions On The Folding Of Silk Fibroin Studied By Generalized Two-Dimensional NMR-NMR Correlation And Raman Spectroscopy

Authors: Qing-Xia Ruan, Ping Zhou, Bing-Wen Hu and Dan Ji

Field: Polymer Science, Macromolecular Science, Analytical Chemistry

Document Summary: This study investigates the influence of potassium ions (K⁺) on the conformational transition of silk fibroin, a key protein in silk fibers. Using generalized two-dimensional NMR-NMR correlation and Raman spectroscopy, the research explores how varying K⁺ concentrations affect the secondary structure of silk fibroin. The findings suggest a sequential change in fibroin structure from helix and/or random coil to helix-like, then to β-sheet-like, and finally to β-sheet as K⁺ concentration increases. This research provides insights into the natural silk-spinning process and offers a theoretical basis for biomimetic spinning, with potential relevance to the aggregation of other β-sheet proteins.

Detailed Table of Contents:

• Keywords
• Correspondence
• Abstract
• Introduction
• Materials and Methods
• Results
• Discussion
• References
• Acknowledgements