Báo cáo Y học: Chemical structure and immunoreactivity of the lipopolysaccharide of the deep rough mutant I-69 Rd–/b+ of Haemophilus influenzae

Chemical Structure And Immunoreactivity Of The Lipopolysaccharide Of The Deep Rough Mutant I-69 Rd¯/B+ Of Haemophilus Influenzae

Authors:

Sven Müller-Loennies, Lore Brade, and Helmut Brade

Field:

Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany

Document Content:

This study investigates the lipopolysaccharide (LPS) of the deep rough mutant I-69 Rd¯/b+ of Haemophilus influenzae. Two oligosaccharides were isolated from de-O-acylated LPS and their structures were determined using NMR spectroscopy. These structures were identified as αKdo-4P-(2→6)-βGlcN-4P-(1→6)-αGlcN-1P and αKdo-5P-(2→6)-βGlcN-4P-(1→6)-αGlcN-1P. The study also confirmed the specificity of existing monoclonal antibodies (mAbs) and assigned the epitope specificity of a new mAb (S42-10-8) to an epitope composed of the bisphosphorylated glucosamine backbone of lipid A and Kdo-4P, with the latter dictating the specificity based on phosphate group position.

Detailed Table of Contents:

• Introduction
• Materials and Methods
• Bacteria and bacterial LPS
• Neoglycoconjugates
• MAbs
• Serology
• Results
• Isolation and structural analysis of the phosphorylated carbohydrate backbone of I-69 LPS
• Serology
• Discussion
• Acknowledgements
• References