Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells

Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells

Tác giả: Elizabeth H. Moore, Mariarosaria Napolitano, Michael Avella, Fatos Bejta, Keith E. Suckling, Elena Bravo and Kathleen M. Botham

Lĩnh vực: Biomedical Sciences, Biochemistry, Pharmacology

Nội dung tài liệu: This study investigates how incorporating the antioxidant probucol into chylomicron remnant-like particles (CRLPs) affects their uptake by human macrophages and the subsequent lipid accumulation within these cells. The research indicates that probucol-containing CRLPs (pCRLPs) are taken up by macrophages at a faster rate compared to CRLPs without probucol. This enhanced uptake, along with an increase in intracellular synthesis of triacylglycerol, leads to a significant rise in lipid accumulation in macrophages. The findings suggest that the protection of chylomicron remnants from oxidation, by antioxidants like probucol, enhances rather than inhibits their role in promoting lipid accumulation in macrophages. This mechanism is proposed as a potential contributor to atherosclerosis development, particularly when the clearance of remnants from circulation is delayed.

Mục lục chi tiết:

• Introduction
• Materials and methods
• Preparation of CRLPs
• Culture of THP-1 cells
• mRNA analysis
• Analytical methods
• Results
• Characteristics of CRLPs and pCRLPs
• Effect of CRLPs containing probucol on lipid accumulation in THP-1 macrophages
• Uptake of CRLPs and pCRLPs by THP-1 macrophages
• Effect of pCRLPs on lipid synthesis in THP-1 macrophages
• Effect of CRLPs containing probucol or lycopene on mRNA expression in THP-1 macrophages
• Discussion
• Acknowledgements
• References