Báo cáo khoa học: The CssRS two-component regulatory system controls a general secretion stress response in Bacillus subtilis

The CssRS two-component regulatory system controls a general secretion stress response in Bacillus subtilis

Authors: Helga Westers, Lidia Westers, Elise Darmon, Jan Maarten van Dijl, Wim J. Quax, and Geeske Zanen

Field: Molecular Biology, Genetics

Document Summary: This study investigates the CssRS two-component regulatory system in Bacillus subtilis, focusing on its role in controlling a general secretion stress response. Previous research indicated that the expression of HtrA and HtrB proteases is linked to secretion stress, triggered by high-level production of secreted α-amylases. This work aimed to determine if membrane translocation of these α-amylases is necessary for this response and if other secretory proteins can also elicit it. The findings demonstrate that the CssRS-dependent response is indeed a general secretion stress response, triggered by both homologous and heterologous secretory proteins. Crucially, membrane translocation of the α-amylase precursor is required to initiate this response. Investigations using lipase A and human interleukin-3 further support the generality of this stress response, though its intensity may not always directly correlate with protein production levels, being influenced by factors like degradation by extracellular proteases.

Detailed Table of Contents:

• Keywords
• Correspondence
• Abstract
• Introduction
• Results
• Discussion
• Experimental Procedures
• Acknowledgements
• References