Báo cáo khoa học: The transcription regulator RbsR represents a novel interaction partner of the phosphoprotein HPr-Ser46-P in Bacillus subtilis

The transcription regulator RbsR represents a novel interaction partner of the phosphoprotein Hpr-Ser46-P in Bacillus subtilis

Authors: Wolfgang Müller, Nicola Horstmann, Wolfgang Hillen, and Heinrich Sticht

Field: Biochemistry, Bioinformatics, Microbiology

Document Content: This study investigates the interaction between the histidine-containing protein (HPr) and transcriptional regulators in Bacillus subtilis. Using a structure-based computational approach combined with experimental validation, the research identifies the ribose operon repressor (RbsR) as a novel interaction partner of HPr, specifically the phosphorylated form HPr-Ser46-P. This finding suggests a broader role for HPr in transcriptional control than previously understood, participating in carbon catabolite repression alongside CcpA.

Detailed Table of Contents:

• Keywords
• Correspondence
• Abstract
• Introduction (Histidine-containing protein (HPr), Carbon catabolite repression, CcpA, HPr-Ser46-P)
• Abbreviations
• Prediction of novel protein-protein interactions
• Results and Discussion
• General outline of the approach used to identify novel protein-protein interactions
• Molecular modeling and interface analysis of the orthologous complexes
• Molecular modeling and interface analysis of the paralogous complexes
• RbsR as a novel interaction partner for HPr-Ser46-P
• Structural comparison of the HPr-CcpA and HPr-RbsR complexes
• Conclusions
• Experimental procedures
• Sequence search and molecular modeling
• Scoring of the protein-protein interfaces
• Cloning
• Protein purification
• EMSA
• Acknowledgements
• References
• Supplementary material