Summary of Doctoral Thesis in Biology: Research on increasinh the accumulation of bioactive alkaloid compound from Eurycoma longifolia Jack breeded on 20- liter bioreactor

Research on Increasing the Accumulation of Bioactive Alkaloid Compounds from Eurycoma Longifolia Jack Breeded on 20-Liter Bioreactor

Tác giả: Tran Thu Trang
Lĩnh vực: Biochemistry
Nội dung tài liệu:
This doctoral thesis focuses on researching methods to increase the accumulation of bioactive alkaloid compounds from Eurycoma Longifolia Jack, specifically utilizing a 20-liter bioreactor system. The study investigates the potential of Eurycoma Longifolia Jack as a medicinal plant, its current over-exploitation, and the challenges in obtaining raw materials for pharmaceutical production. It explores the application of in vitro culture techniques, particularly hairy root biomass culture in bioreactors, as a viable solution for stable and efficient production of valuable compounds. The research aims to optimize culture conditions, evaluate the impact of elicitors on alkaloid accumulation, determine the chemical structure and biological activities of isolated alkaloids, and establish a quantitative method for their analysis. The thesis contributes to understanding and enhancing the production of bioactive compounds from Eurycoma Longifolia Jack through advanced biotechnology.
Mục lục chi tiết:
CHAPTER I. OVERVIEW
1.1. Introduction of Eurycoma Longifolia
1.2. Application of Eurycoma Longifolia in Traditional Medicine
1.3. Hairy roots biomass culture
1.3.1. Introduction of hairy roots biomass culture
1.3.2. Factors affecting the ability to synthesize HCTC in hairy root culture
1.3.3 Effect of elicitor on accumulation of HCTC during hairy root culture
1.3.4 The situation of domestic and foreign research on hairy root biomass culture receiving financial credits
CHAPTER II: MATERIALS AND METHODS
2.1. Research materials
2.2. Research Methods
2.2.1. Method of isolating and determining the chemical structure of alkaloids from hairy root biomass.
Methods of determining chemical structure
2.2.2. In vitro biological activity research methods
2.1.2.1 Cytotoxic assay (cytotoxic assay)
2.1.2.2 Testing the ability to inhibit the production of IL-6 and TNF-a in mouse and human macrophages stimulated by LPS of substances (1), (2), (3)
2.2.3 Experimental and quantitative methods of alkaloids through HPLC-DAD method.
2.2.4. Evaluate factors affecting the growth and accumulation of alkaloids of hairy root of E.longifolia in 500 ml flask.
2.2.5. Establishing the process of cultivating and collecting E.longifolia hairy root biomass on 20-liter Bioreactor system
CHAPTER III: RESULTS AND DISCUSSION
3.1. Determine the chemical structure of substances isolated from alkaloid extract
3.2. Biological activity of 7-MCPA, 9-methoxycanthin-6-one, 9-hydroxycanthin-6-one
3.2.1. Carcinogenicity of the test substances (1), (2), (3)
3.2.2. Testing the ability to inhibit the production of IL-6 and TNF-a in mouse and human macrophages stimulated by LPS of substances (1), (2), (3)
3.3. Constructing a quantitative calibration curve for alkaloids in HPLC-DAD methods