Báo cáo khoa học: Lumican expression is positively correlated with the differentiation and negatively with the growth of human osteosarcoma cells

LUMICAN EXPRESSION IS POSITIVELY CORRELATED WITH THE DIFFERENTIATION AND NEGATIVELY WITH THE GROWTH OF HUMAN OSTEOSARCOMA CELLS

Authors: Dragana Nikitovic, Aikaterini Berdiaki, Alexandros Zafiropoulos, Pavlos Katonis, Aristidis Tsatsakis, Nikos K. Karamanos, George N. Tzanakakis

Field: Histology, Orthopaedics, Biochemistry

Document Content: This study investigates the expression and functional role of lumican, a small leucine-rich proteoglycan, in human osteosarcoma cell lines. The research examines lumican’s correlation with cell differentiation and growth, utilizing moderately differentiated (MG-63) and well-differentiated (Saos 2) osteosarcoma cell lines. Methods employed include real-time PCR, western blotting, and specific enzymatic digestions. The findings indicate that lumican expression is significantly higher in well-differentiated Saos 2 cells compared to highly metastatic MG-63 cells. Lumican was found to inhibit the growth of Saos 2 cells while promoting their migration and chemotactic response. Conversely, lumican’s impact on MG-63 cells was less pronounced. The study suggests a positive correlation between lumican expression and cell differentiation, and a negative correlation with osteosarcoma progression.

Detailed Table of Contents:

• Keywords
• Correspondence
• Abstract
• Abbreviations
• Introduction
• Results: Human osteosarcoma cell lines express lumican mRNA and efficiently secrete lumican protein substituted to cell culture medium
• Results: Lumican mRNA levels were efficiently down-regulated following transfection with siRNA
• Results: Effect of lumican siRNA on cell proliferation
• Results: Effect of lumican down-regulation on Smad signalling
• Results: Effect of lumican down-regulation on p21WAF-1 expression
• Results: Effect of lumican siRNA on cell migration and chemotaxis-induced motility
• Discussion
• Experimental procedures: Reagents
• Experimental procedures: Cell cultures
• Experimental procedures: Protein isolation and enzymatic digestion
• Experimental procedures: RNA isolation and real-time PCR
• Experimental procedures: SDS-PAGE and immunoblotting
• Experimental procedures: Transfection experiments
• Experimental procedures: Proliferation assays
• Experimental procedures: Wound healing assay
• Experimental procedures: Chemotaxis cell migration assay
• References