Xem trước tài liệu

Đang tải tài liệu...

Thông tin chi tiết tài liệu

Định dạng: PDF
Số trang: 12 trang
Dung lượng: 236 KB

Giới thiệu nội dung

H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae

Authors: Hugo Osorio, Elisabete Carvalho, Mercedes del Valle, María A. Günther Sillero, Pedro Moradas-Ferreira, Antonio Sillero

Field: Biochemistry, Molecular Biology

Document Description: This study investigates the effects of hydrogen peroxide (H2O2) and menadione on nucleotide metabolism in Saccharomyces cerevisiae. The research observed that H2O2 treatment significantly decreased ATP levels and increased inosine levels, while menadione had a less pronounced effect. The findings suggest that H2O2 may inhibit glycolysis, leading to these nucleotide changes. The study also explored the resistance of yeast cells to H2O2 when grown on different carbon sources, such as glycerol or ethanol, compared to glucose or galactose. Through experimental analysis and theoretical modeling, the researchers aimed to elucidate the mechanisms underlying the cellular response to oxidative stress.

Detailed Table of Contents:

  • Introduction
  • Materials and methods
  • Strain and growth conditions
  • H2O2 and menadione treatment: control of cell viability
  • Extraction of nucleosides and nucleotides
  • Energy charge
  • Preparation of cell extracts
  • Enzymatic assays
  • Results
  • Effect of H2O2 on the nucleotide content of yeast cells, grown in the presence of galactose, glucose or mannose
  • Effect of menadione on the nucleotide content of yeast cells
  • Effect of H2O2 on yeast cells growing in the presence of glycerol or ethanol
  • Search for a plausible mechanism
  • Experimental approach – determination of enzyme activities related to adenine metabolism
  • Theoretical approach – mathematical simulation of some metabolic pathways related to adenine nucleotide metabolism
  • Effect of H2O2 on glycolysis
  • Recovery of yeast cells after the oxidative stress caused by H2O2
  • Discussion
  • Acknowledgements
  • References