Báo cáo khoa học: Acoustic microfluidic chip technology to facilitate automation of phage display selection

Acoustic Microfluidic Chip Technology to Facilitate Automation of Phage Display Selection

Authors:

Jonas Persson, Per Augustsson, Tomas Laurell, Mats Ohlin


Affiliation:

Department of Immunotechnology, Lund University, Sweden; Department of Electrical Measurements, Lund University, Sweden


Field:

Biotechnology, Proteomics, Microfluidics


Abstract:

This study introduces an acoustic microfluidic chip technology designed to automate the selection and separation of specific binders from phage display libraries. The system utilizes acoustic standing wave forces to efficiently separate antigen-bound from unbound phage particles in a continuous flow format. This innovation addresses a critical bottleneck in high-throughput proteome analysis, offering a flexible and automatable solution for isolating diverse and specific antibody fragments. The developed chip-based system demonstrates efficiency comparable to conventional methods, with the potential for significant throughput and minimal manual intervention, paving the way for automated unit operations in binder development.


Keywords:

acoustic standing wave forces, antigen-specific binding, microfluidic chip, phage display, selection