Structural Analysis of the Lipopolysaccharide from Nontypeable Haemophilus influenzae Strain 1003

Structural Analysis of the Lipopolysaccharide from Nontypeable Haemophilus influenzae Strain 1003

Tác giả: Martin Månsson, Derek W. Hood, Jianjun Li, James C. Richards, E. Richard Moxon, and Elke K. H. Schweda

Lĩnh vực: Biochemistry, Microbiology

Nội dung tài liệu: This document details the comprehensive structural analysis of lipopolysaccharide (LPS) from the nontypeable Haemophilus influenzae strain 1003. Utilizing advanced techniques including high-field NMR, ESI-MS, and capillary electrophoresis coupled to ESI-MS, the study elucidates the intricate composition and linkage of the LPS molecule. The research identifies the conserved structural elements of H. influenzae LPS, specifically the O-deacylated lipid A and core oligosaccharide, and characterizes the various glycoforms present. The findings also highlight the presence of common substituents like phosphocholine and N-acetylneuraminic acid, contributing to the understanding of LPS heterogeneity and its implications for bacterial virulence and host immune evasion.

Mục lục chi tiết:

• Introduction
• Experimental Procedures
• Bacterial cultivation and preparation of LPS
• Chromatography
• Preparation of oligosaccharides
• Mass spectrometry
• NMR spectroscopy
• Results
• Characterization of LPS
• Characterization of oligosaccharides
• Characterization of LPS-OH and OS-1 by NMR
• Discussion
• Acknowledgements
• References