Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer

Constitutive Oligomerization of Human D₂ Dopamine Receptors Expressed in Spodoptera frugiperda 9 (Sf9) and in HEK293 Cells

Authors:

Lucien Gazi, Juan F. López-Giménez, Martin P. Rüdiger, Philip G. Strange

Field:

Biochemistry, Pharmacology

Document Content:

This study investigates the constitutive oligomerization of human D₂ dopamine receptor isoforms (D₂Long and D₂Short) in Spodoptera frugiperda 9 (Sf9) and HEK293 cells. Utilizing co-immunoprecipitation and time-resolved fluorescence resonance energy transfer (FRET), the research demonstrates that both D₂L and D₂s receptors form homo-oligomers and hetero-oligomers in Sf9 cells. The study also provides evidence for constitutive oligomerization of D₂s receptors in HEK293 cells. Notably, the findings indicate that D₂ receptor ligands do not significantly affect this oligomerization process in either cell system. The research highlights the applicability of the Sf9 cell system for analyzing receptor pharmacology and oligomerization, and suggests that D₂ receptor isoform hetero-oligomerization may play a crucial role in receptor function, potentially influencing the efficacy of antipsychotic drugs.

Detailed Table of Contents:

• Introduction
• Materials and Methods
• Results
• Discussion
• Acknowledgements
• References